medicine leaf
pointing to tube
man with bioreactor
157_9_GLP6_Hood_2-1
BIOSC 157/9: Foundations In Biotechnology

Good Laboratory Practice ( lab notebook, technical skill, improving SOP's)

186_1_BIoreactor_JavierBioreact
BIOSC 186 : Protein Purification and Analysis

Bioreactor use (grow and sample cells in carefully controlled aseptic conditions)

1_diagnostic_dreamstime_xxl_204427424
BIOSC 182 GLP and cGMP, Principles and Compliance

What are the penalties for failing? (right to operate, warning letters, recall)

157_2_MeasuringCintiaRJGerald
BIOSC 157/9: Foundations in Biotechnology

Precision measuring devices (micropipettes, analytic balance, spectrophotometry)

186_2_Column_BijonJacobSyrings
BIOSC 186 : Protein Purification and Analysis

Column chromatography (ACTA-FPLC, size exclusion, ion-exhange)

2_pharmaceutical_dreamstime_xxl_9966528
BIOSC 182 GLP and cGMP, Principles and Compliance

What is regulated (cannabis, pharmaceutical, diagnostic testing, GMO's)

157_3_LabCalcGeraldDeangelaHappyNotebookPlant-1
BIOSC 157/9 Foundations in Biotechnology

Lab calculations (dilutions, standard curves, solution recipes)

186_3_IsolationKatie_Javier
BIOSC 186 : Protein Purification and Analysis

Protein isolation (extraction technique, separation, storage, labeling)

3_cannabis_dreamstime_xxl_169891901
BIOSC 182 GLP and cGMP, Principles and Compliance

Changes to regulations? (legislation, advocacy, critical thinking)

157_4_AsepticGroupWeighSoil-1
BIOSC 157/9 Foundations in Biotechnology

Aseptic technique (biosafety cabinets, media, avoid sample contamination)

186_4_BiologicalELISA_PLate
BIOSC186 : Protein Purification and Analysis

Biological assays (HPLC, FPLC to detect contaminants and impurities)

4_authorities_dreamstime_xxl_181708099
BIOSC 182 GLP and cGMP, Principles and Compliance

Who are the authorities? (FDA, CA Dept of Cannabis control, USDA, EPA, DEA)

157_5_GMPTissueCultureGroup-1
BIOSC 157/9 Foundations in Biotechnology

cGMP (following SOP's, batch recrods, validation)

186_5_ScaleUpJavier_JacobSample
BIOSC 186 : Protein Purification and Analysis

Scale up (test multiple growth conditions, select most productive)

5_medicalcann_dreamstime_xxl_78474235
BIOSC 182 GLP and cGMP, Principles and Compliance

Why do the regulations exist? (history of legislation, enforcement, societal effects)

157_6_LoadGel
BIOSC 157/9 Foundations in Biotechnology

Bioseparations (HPLC, FPLC, agarose gel, centrifiguation)

186_6_Analysisglowtubes1
BIOSC 186 : Protein Purification and Analysis

Analysis of purity data (prepare certificate of analysis, active ingredient, contaminant/impurity levels)

6_rules_dreamstime_xxl_15151948
BIOSC 182 GLP and cGMP, Principles and Compliance

What are the rules? (cGMP, SOP's, Certificate of analysis, documentation)

157_7_Datainterp_ComputerShare1-1
BIOSC 157/9 Foundations in Biotechnology

Data interpretation and analysis (excel, statistics, graphing)

186_7_CalculateAlbert_DeAngela_Jacob
BIOSC 186 : Protein Purification and Analysis

Calculate yield (efficiency of process, continuous improvement)

7_comply_dreamstime_xxl_208818777
BIOSC 182 GLP and cGMP, Principles and Compliance

How do we comply? (licensing, training, equipment, procedures, cGMP)

157_8_LabsolnPourPlates1
BIOSC 157/9 Foundations in Biotechnology

Laboratory solutions (growth media, extraction buffers, assays)

186_8_SolutionGradCyllinder
BIOSC 186 : Protein Purification and Analysis

Solution prepration (calculations, precision measurment, sterilization)

8_compliance
BIOSC 182 : GLP and cGMP, Principles and Compliance

How is compliance and safety checked? (audits, applications, documentation, facilities)

157_2_PrecisionEricScale
BIOSC 157/9 Foundations in Biotechnology

Precision measuring devices (micropipettes, analytic balance, spectrophotometry)

186_9_GMP7_Hood_Team_Prep_IMG_8164
BIOSC 186 : Protein Purification and Analysis

cGMP, GLP (batch records, sampling, documentation, acurate technique)

9_safe_dreamstime_xxl_210282170
BIOSC 182 GLP and cGMP, Principles and Compliance

How do I know my medicine is safe? (Biopharmaceutical, Cannabis)

157_10_MicroGabyMicroscope-1
BIOSC 157/9 Foundations in Biotechnology

Microscopy (sample preparation, staining, analysis)

186_10_PrecisionMultiChannelHoodTwo2
BIOSC 186: Protein Purification and Analysis

Precision measuring devices (micropipettes, spectrophotometry, analytical balance)

10_new_dreamstime_xxl_164840097
BIOSC 182 GLP and cGMP, Principles and Compliance

How is a new drug approved? (Canabis, pharmaceutical, etc.)

157_1_DNATechRayAshleyPoint-1
BIOSC 157/9 Foundations in Biotechnology

DNA technology (PCR, extraction, Next generation sequencing)

186_Main_BijonBioreact
BIOSC 186 : Protein Purification and Analysis
previous arrowprevious arrow
next arrownext arrow
Shadow

The field of biotechnology (a field of applied biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine, and other applications) touches nearly every aspect of each of our lives. This program trains and educates students for thoughtful, productive work in research laboratories and industrial situations. Upon completion of a certificate or degree, students will be able to: maintain a safe and productive work environment, manage and communicate information, perform mathematical manipulations, perform measurements/ tests/ assays, prepare solutions and media, and comply with applicable regulations and standards.

Course Requirements (9 major courses for 27 units plus general education for a total of 60 units)

Core Biotechnology Course #1

  • BIOSC172 – Introduction to biotechnology (3U)

Core Biotechnology Course #2

  • BIOSC172L – Introduction to biotechnology lab (1U)

Core Biotechnology Course #3

Core Biotechnology Course #4

  • BIOSC147 – Molecular and Cellular biology (4U)

Core Biotechnology Course #5

  • CHEM120 – General College Chemistry I (5U)

Advanced Lab Course #1 (choose one)

  • BIOSC186 – Purification and analysis of biological molecules (Protein Purification and Analysis) (2U)
  • BIOSC188 – Biotechnology Operations – Analytic Laboratory / Bioprocessing (2U)
  • BIOSC183 – Mammalian Cell Culture (Eukaryotic cell culture) (2U)
  • BIOSC182 – Safety, efficacy, and quality in regulated products (GLP and GMP; Principles and Compliance) (2U)

Advanced Lab Course #2 (choose a second one)

  • BIOSC186 – Purification and analysis of biological molecules (Protein Purification and Analysis) (2U)
  • BIOSC188 – Biotechnology Operations – Analytic Laboratory / Bioprocessing (2U)
  • BIOSC183 – Mammalian Cell Culture (Eukaryotic cell culture) (2U)
  • BIOSC182 – Safety, efficacy, and quality in regulated products (GLP and GMP; Principles and Compliance) (2U)

Business and communication (choose 1)

  • BUS120 – Introduction to Management (3U)
  • SPCH120 – Public speaking

Computer and data proficiency (choose 1)

  • MATH164 – Introduction to Probability and Statistics (4U)
  • CIS135 – Introduction to Computers (4U)
  • BOT224 – Microsoft Excel for Business (3.5U)
  • BOT226 – Microsoft Access for Business (3.5U)

General Education and Electives (to 60 units total)

  • Language and Rationality
  • Oral Communication and Critical Thinking
  • Natural Science
  • Arts and Humanities
  • Social Sciences
  • Mathematics Proficiency

Competencies

  • Graduates have a solid foundation in the basics of biology and an understanding of the breadth of the biotech industry. A.S. degree holders likely have the strongest biology and science background, including the typical lower-level science requirements for biology majors.
  • Graduates are enthusiastic about the field and have the courage and ability to think critically about their projects. They are willing to both explore available information independently and also direct questions to appropriate co-workers when solving problems and improving efficiencies in their work.
  • Graduates always ask about safety considerations when learning a new protocol, understand what typical hazard symbols mean, and are trained to work safely and productively at all times. Action item is to include this in pre-lab requirements

Skills in

  • Provide routine facility (media prep, etc.) support
  • Strong technical skills (precision in pipetting, weighing, sample handling, avoid contamination)
  • Proper use and care of laboratory equipment for cell culture (microscopes, centrifuges, incubators, laminar flow hood, hemocytometers)
  • good documentation practices
  • Items identified appropriately and trackable
  • Dilutions
  • Graphing
  • Test-specific math (analysis, statistics, standard curve)
  • Aseptic technique

Ability to

  • Assess the importance of corrective action and preventive action in continuous improvement
  • Clean and /or sterilize laboratory and supplies
  • Ability to work in a regulated environment
  • Critical thinking
  • Maintain a safe and productive work environment
  • Maintain laboratory notebook and associated documentation
  • Manage and communicate information
  • Perform calculations for solution preparation, separation methods, and data analysis
  • Perform measurements/tests/assays
  • Comply with applicable regulations and standards
  • Perform measurements/tests/assays

Knowledge of

  • scientific method to test and modify ideas about our understanding of molecular biology concepts
  • terminology and acronyms applicable to the field
  • Critical examination and analysis of data
  • performance of a basic bio-separation technique such as column chromatography
  • standard laboratory techniques
  • quality testing: Sampling, documentation, extraction, assay, regulations
  • safety testing: microbial and contaminant assays

What type of jobs does this prepare for?

  • QA/QC Analyst/ Technician/ Associate
  • Laboratory Operations Specialist /Technician
  • Research Associate
  • Biomanufacturing Technician

For the official Associate of Science Degree in Biotechnology Program Outline of Record (POR) click here.